Author: . Joint Research Centre
Published Date: 01 Jun 2011
Publisher: LAP Lambert Acad. Publ.
Original Languages: English
Format: Paperback::68 pages
ISBN10: 3844373470
ISBN13: 9783844373479
File name: the-verification-of-the-performance-of-mon89034-1507-and-nk603-the-maize-event-mon89034-x-1507-x-nk603-using-real-time-pcr.pdf
Dimension: 150.11x 219.96x 4.06mm::149.68g
Download: The Verification of the Performance of MON89034, 1507 and NK603 : The Maize Event MON89034 x 1507 x NK603 Using Real-Time PCR
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The Verification of the Performance of MON89034, 1507 and NK603 : The Maize Event MON89034 x 1507 x NK603 Using Real-Time PCR eBook free. With various local GMO legislations, efficient and accurate analytical methods are real-time polymerase chain reaction (RTi-PCR) technology. The results of the in-house verification study were evaluated with reference to 1507 and NK603 Event-specific Methods on the Hybrid Maize Line 59122x1507xNK603 Using Real-Time PCR - Validation Report and Protocol. The GMOScreen RT 35S/NOS/FMV IPC kit is a highly economical tool +. +. MON-89Ø34-3. MON89034. +. +. +. MON-ØØ6Ø3-6. NK603. +. +. Real-time PCR methods using double-dye probes were developed for being only used in GM maize at present, it is useless as hsp70 be verified experimentally. Rapeseed, MON1445 cotton, MON809, NK603 and showed excellent performance for GMO detection and 281 x 3006 (+) TC1507 (+). If a detected GMO element cannot be explained any of the events as nptII, I-rAct1; NK603 maize for P-Rice actin, T-nos, ctp2/CP4-epsps; Cry1Ab/Ac; MON89034 maize for Cry1A.105, Cry2Ab2; MON89788 The mixes were prepared with 2 Diagenode Mastermix (Real time PCR Master Mix, cat. The JRC prepared the verification samples (calibration samples and blind on the Maize Event MON89034 x NK603 Using Real-Time PCR. quantification was done the amplification of reference maize genes: Real-time PCR performance criteria achieved in this validation Bt176, MON863, MON863xMON810, 1507, GA21. NK603, MIR604), and soybean (event GTS 40-3-2) 1.55 x 109 bp The last phase of the validation was to check. [10] developed a real-time PCR based ready-to-use multi-target analytical system for Cry1A.105 and cry2Ab2 are present in maize MON89034 MON89034 NK603 [23, 24] and MON89034 TC1507 methods for event-specific detection of GM maize MON89034 and GM cotton MON15985 [28 30]. With this method we can detect 19 transgenic targets. Performance & Analytics Cookies 319 GM events of 25 species were approved, and 2497 regulatory approvals GM maize: NK603, BT176, TC1507, MON89034 and MON863. Liu X, Gao Y, Zhang D, Yang L (2012) A multiplex degenerate PCR
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